Gyrolab Spin Blog

May 22, 2021 11:15:00 AM

Accurate ligand affinity determination is crucial when targeting protein-protein interactions

Johan Engström, Sr Scientist at Gyros Protein Technologies

Part 2: Determining the dissociation constant (KD) for a high-affinity Adnectin imaging ligand by in-solution affinity measurement

Determining the dissociation constant (KD) for a high-affinity Adnectin™ imaging ligand by in-solution affinity measurementAnalyzing high-affinity ligands used to target protein-protein interactions can be a real challenge. A biotherapeutics discovery team at Bristol Myers Squibb (BMS) used Gyrolab® in-solution affinity analysis to overcome problems in determining the picomolar-level dissociation constant of an imaging Adnectin™ ligand. Advantages also included low sample consumption, high throughput, and more accurate values for low-picomolar dissociation constants than surface plasmon resonance (SPR).

 

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Topics: Affinity determination


May 21, 2021 8:23:57 AM

Accurate ligand affinity determination is crucial when targeting protein-protein interactions

Johan Engström, Sr Scientist at Gyros Protein Technologies

Part 1: High-affinity ligands for imaging target expression in cancer immunotherapy

The value of high-affinity ligands targeting PPIs in cancer immunotherapy-1

Cancer immunotherapy targeting protein-protein interactions saves lives. Success builds on the binding characteristics of high-affinity binding therapeutic antibodies and also ligands used to monitor target expression during clinical trials. Measuring nano- to low-picomolar affinity binding can be difficult using surface plasmon resonance (SPR), which led a biotherapeutics discovery team to choose in-solution affinity analysis in the characterization a high-affinity imaging ligand.

 

 

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Topics: Affinity determination