Johan Engström, Sr Scientist at Gyros Protein Technologies
Part 2: Determining the dissociation constant (KD) for a high-affinity Adnectin™ imaging ligand by in-solution affinity measurement
Analyzing high-affinity ligands used to target protein-protein interactions can be a real challenge. A biotherapeutics discovery team at Bristol Myers Squibb (BMS) used Gyrolab® in-solution affinity analysis to overcome problems in determining the picomolar-level dissociation constant of an imaging Adnectin™ ligand. Advantages also included low sample consumption, high throughput, and more accurate values for low-picomolar dissociation constants than surface plasmon resonance (SPR).