PurePrep Blog - symphony-x

New White Paper: Meeting the challenge of synthesizing complex therapeutic peptides

Sep 1, 2020 9:52:00 AM

The demand for increasingly complex therapeutic peptides has resulted in the development of robust, efficient, and rapid synthesis protocols and instrumentation that can meet the demands of GxP and regulatory authorities. Read more about synthesizing complex therapeutic peptides.

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Topics: Symphony X, Peptide synthesis, PurePep Chorus, Therapeutic peptides

Fluorescent labeling of a peptide toxin retains the specificity and affinity required of a research tool

Aug 20, 2020 9:51:00 AM

Peptide toxins from animals are potent inhibitors of ion channels and show great promise both as drugs to treat a range of disorders and as valuable tools in drug research. Utilizing Symphony® X peptide synthesizer from Gyros Protein Technologies, a research team based in France has explored ways of fluorescently labeling a scorpion peptide, BeKm-1, that inhibits the hERG ion channel. This channel is involved in cardiac activity and blocking it must be avoided when developing new drugs. The research team’s approach included protein-protein docking studies that enabled them to identify solvent-exposed residues in the peptide that were suitable for labeling. This enabled them to design and test analogs that maintained both specificity and mode of action of the native peptide.

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Topics: Symphony X, Peptide synthesis, Peptide synthesizer, Peptide toxins

5 factors to consider when choosing a peptide synthesizer

Apr 24, 2019 11:00:00 AM

Choosing a peptide synthesizer to meet your specific needs can be a daunting task. Here are some key factors to consider to be able to synthesize high quality peptides in the number and scale that matches your applications.

1. The question of throughput and parallel synthesis

Different applications and phases of research require different numbers of peptides. A peptide drug discovery program may involve screening hundreds (or even thousands) of peptides to find hits, followed by optimizing structure-activity relationships (SAR) with focused libraries to identify a single candidate that can be advanced to the clinic. The key to success is therefore to choose a synthesizer that has reaction vessels that can deliver the level of parallel synthesis you need to match your application1.

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Topics: Prelude x, Symphony X, Peptide synthesis, Tribute, SPPS, Sonata XT, Real-time UV monitoring

Inhibitory peptides help identify receptor complex vital to male fertility

Mar 12, 2018 9:00:00 AM

The acrosome reaction is critical for the sperm to penetrate the female egg and fertilize it. Progesterone plays a key role in the acrosome reaction, but how this works has been unclear since spermatozoa lack classical progesterone receptors. Wenming Xu and collaborators at Sichuan University, China, The Chinese University of Hong Kong, and the Australian National University, have shown that the progesterone-induced Ca2+ influx required for the acrosome reaction involves the progesterone receptor or modulator, gamma-aminobutyric acid type A (GABAA) receptor delta subunit (GABRD), in combination with the P2X2 receptor.
The first confirmation that delta subunits were involved in the Ca2+influx came with an experiment that demonstrated that increased intracellular levels of Ca2+ in spermatozoa after progesterone stimulation were suppressed by the δ(392-422) inhibitory peptide but unaffected by a scrambled δ control peptide or a Υ2-peptide.

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Topics: Symphony X, Peptide synthesis, Male fertility, The acrosome reaction, Inhibitory peptides

Designer peptoids-improving on Mother Nature

Jul 27, 2017 10:00:00 AM

Peptoids offer a way of improving on nature in a range of biomedical applications such as antimicrobial agents, nanomaterials and diagnosis and treatment of a wide number of cancers.Over 150 peptoid-related papers are now being published per year, and in total, there are over 1700 publications.

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Topics: Symphony X, Peptide synthesis, Peptoids