Fluorescent labeling of a peptide toxin retains the specificity and affinity required of a research tool

Aug 20, 2020 9:51:00 AM

Peptide toxins from animals are potent inhibitors of ion channels and show great promise both as drugs to treat a range of disorders and as valuable tools in drug research. Utilizing Symphony® X peptide synthesizer from Gyros Protein Technologies, a research team based in France has explored ways of fluorescently labeling a scorpion peptide, BeKm-1, that inhibits the hERG ion channel. This channel is involved in cardiac activity and blocking it must be avoided when developing new drugs. The research team’s approach included protein-protein docking studies that enabled them to identify solvent-exposed residues in the peptide that were suitable for labeling. This enabled them to design and test analogs that maintained both specificity and mode of action of the native peptide.“Symphony X is a valuable addition to our synthetic capabilities here at Smartox. As a long-time user of GPT products we not only rely on the instrument’s robust construction and reliability, but also the increased flexibility this model offers when producing peptides with non-standard amino acids and different chemistries. Being able to synthesize multiple peptide analogs simultaneously is a huge advantage, allowing us to increase efficiency and productivity for our peptide laboratory.”

Dr. Michel De Waard, CSO Smartox Biotechnology and INSERM senior research director

 

 

Read the complete summary in the PDF

 

Topics: Symphony X, Peptide synthesis, Peptide synthesizer, Peptide toxins