PurePrep Blog

Peptide vaccine development and production

Nov 4, 2020 5:42:09 PM

Vaccines are often regarded as the most important biomedical advance in the fight against infectious disease. The ability of vaccines to elicit a protective immune response is also being exploited in the fight against cancer and Alzheimer’s disease. While conventional vaccines against infectious diseases are based on inactivated or live attenuated pathogens, peptide-based vaccines have many advantages, including fully-defined composition with no biological contamination, the possibility to customize, and the possibility of cost-effective large-scale production. Success in the rational design, development and production of peptide vaccines depends on many factors, not least the ability to synthesize often complex peptides efficiently and quickly.

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The Power and the Promise of Peptide Therapeutics

Nov 4, 2020 5:00:07 PM

Thousands of peptides are produced in the body to perform a wide range of functions. This makes peptides a promising route toward well-tolerated and effective treatments. Therapeutic peptides are now being used to treat a range of conditions, including metabolic diseases, cancer, cardiovascular and infectious diseases, pain and hematological diseases. This has been the result of considerable efforts in R&D, including the development of advanced methods for solid-phase peptide synthesis to prepare customized peptides with improved function, and greater resistance to degradation.

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Topics: Peptide synthesis

How to Ensure Optimal Peptide Purity & Yield in SPPS

Nov 4, 2020 4:51:59 PM

How to ensure optimal purity and yield in solid-phase peptide synthesis

Previous articles in this series have highlighted how peptide synthesis has enabled the development of a wide range of powerful vaccines and therapeutic agents. This has been made possible by the ability to synthesize peptides to high purity and in large scale. An almost limitless combination of sequences that often include modified amino acids, however, makes considerable demands on synthesis optimization.

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Topics: Peptide synthesis

New White Paper: Meeting the challenge of synthesizing complex therapeutic peptides

Sep 1, 2020 9:52:00 AM

The demand for increasingly complex therapeutic peptides has resulted in the development of robust, efficient, and rapid synthesis protocols and instrumentation that can meet the demands of GxP and regulatory authorities. Read more about synthesizing complex therapeutic peptides.

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Topics: Symphony X, Peptide synthesis, PurePep Chorus, Therapeutic peptides

Fluorescent labeling of a peptide toxin retains the specificity and affinity required of a research tool

Aug 20, 2020 9:51:00 AM

Peptide toxins from animals are potent inhibitors of ion channels and show great promise both as drugs to treat a range of disorders and as valuable tools in drug research. Utilizing Symphony® X peptide synthesizer from Gyros Protein Technologies, a research team based in France has explored ways of fluorescently labeling a scorpion peptide, BeKm-1, that inhibits the hERG ion channel. This channel is involved in cardiac activity and blocking it must be avoided when developing new drugs. The research team’s approach included protein-protein docking studies that enabled them to identify solvent-exposed residues in the peptide that were suitable for labeling. This enabled them to design and test analogs that maintained both specificity and mode of action of the native peptide.

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Topics: Symphony X, Peptide synthesis, Peptide synthesizer, Peptide toxins

Designing peptides to meet the challenge of the next generation of druggable targets

Jul 30, 2020 9:49:00 AM

Targeting protein–protein interactions (PPIs) is a new challenge in expanding druggable space. Cyclic peptides show promise in targeting PPIs and to better understand their structure–activity relationships a research team at Tufts University, USA, has analyzed a series of designed, well-structured cyclic hexapeptides and use simulations and experimental techniques to understand their global structural ensembles. They discovered a previously unappreciated role for β-branched residues in stabilizing specific conformations of cyclic hexapeptides, and their approach shows promise in the prediction of structure–activity relationships for drug development. Their work was rewarded by being on the front cover of Biophysical Journal, Volume 116, Issue 3.

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Topics: Peptide synthesis, Protein-Protein Interactions (PPIs), Tribute peptide synthesizer, Cyclic peptides

Peptide inhibitor of methylation casts new light on epigenetic-based cancer therapy

Jul 1, 2020 12:00:00 AM

The first peptide-derived epigenetic drug (Romidepsin) was approved in 2009 for the treatment of cutaneous T cell lymphomas (CTCL), and in 2011 for peripheral T cell lymphomas (PTCL) (2). Romidepsin also has potential in the treatment of other kinds of cancer, lung fibrosis, and Epstein-Barr infections. Currently, it is the only FDA-approved peptide medicine specifically designed to target epigenetic effects. Nesiritide, a peptide originally approved in 2001 for the symptomatic treatment of acute decompensated heart failure due to its vasodilating activity has since been repurposed due to its epigenetic effects. These peptides are not yet approved in the European Union by the EMA.

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Topics: Prelude, Peptide synthesis, Protein-Protein Interactions (PPIs), Peptide synthesizer, Cyclic peptides

Unlocking aggregation in amyloid peptides opens up Alzheimer’s research

Sep 30, 2019 9:00:00 AM

Alzheimer's is a progressive degenerative disease of the brain that accounts for 60–80% of dementia cases, which cover a range of cognitive disabilities including memory loss (1). Alzheimer's is the sixth leading cause of death in the United States, with an expected life expectancy of 4–8 years after diagnosis.This disease is not a normal part of aging, and approximately 200,000 Americans under the age of 65 have younger-onset Alzheimer’s disease. There is currently no cure, but symptoms can be treated and great efforts are being made worldwide to improve treatment, delay onset, and ultimately prevent the disease from developing.The amyloid beta peptides involved in Alzheimer’s disease are prone to aggregation, challenging both synthesis and purification. A group based in Auckland New Zealand therefore developed a method to reversibly introduce double linkers into the synthetic peptide that reduce aggregation and improve solubility, resulting in higher yields and purity. 

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Topics: Peptide synthesis, Alzheimer's disease, Tribute peptide synthesizer, SPPS, Amyloid peptides, Pseudoproline

Overcoming opioid side effects with combination peptide therapy

Aug 20, 2019 8:30:00 AM

Unrelieved pain is a major global healthcare problem. There are two types of pain: acute pain, which is typical after surgery and trauma and chronic or recurring pain, which is common in individuals suffering from osteo- and rheumatoid arthritis, those with spine problems, and can be developed after operations and injuries. The problem is so great that chronic and recurrent pain is regarded as a disease in its own right. Apart from the burden of suffering, chronic pain results in a considerable cost to society that is on a par with cancer or cardiovascular disease by affecting one in five adults.

Opioids are the analgesics most prescribed to treat moderate to severe pain but their use leads to a number of debilitating side effects, diminishing efficacy, and also the risk of abuse and misuse. A research team at Université de Sherbrooke in Canada has investigated combining the prototypical opioid morphine and an analgesic neurotensin brain-penetrant peptide analog to treat pain. They discovered that co-administration resulted in an additive analgesic response that permitted the dosing of morphine to be reduced and also decreased the level of a typical adverse side effect, constipation.

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Topics: Peptide synthesis, SPPS, Symphony peptide synthesizer, Combination peptide therapy, Pain treatment

5 factors to consider when choosing a peptide synthesizer

Apr 24, 2019 11:00:00 AM

Choosing a peptide synthesizer to meet your specific needs can be a daunting task. Here are some key factors to consider to be able to synthesize high quality peptides in the number and scale that matches your applications.

1. The question of throughput and parallel synthesis

Different applications and phases of research require different numbers of peptides. A peptide drug discovery program may involve screening hundreds (or even thousands) of peptides to find hits, followed by optimizing structure-activity relationships (SAR) with focused libraries to identify a single candidate that can be advanced to the clinic. The key to success is therefore to choose a synthesizer that has reaction vessels that can deliver the level of parallel synthesis you need to match your application1.

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Topics: Prelude x, Symphony X, Peptide synthesis, Tribute, SPPS, Sonata XT, Real-time UV monitoring